A “Paper Machine” for Molecular Diagnostics

The cost of the device, as shown in Figure 1, was estimated using low-volume pricing for all materials and reagents (Table S1). Once scaled-up for high-volume manufacturing, bulk discounts would further decrease the total cost. The transition from 25 µL LAMP reactions in tubes, to 10 µL reactions run in FTA paper, required optimization of the reaction conditions. Here, we investigated two different versions of the polymerase, the incubation time, and varying concentrations of betaine and the primers (sequences shown in Table S2).